Day 1 Part 1:
Prepare Log Phase Bacteria
Ingredients:
20 microliters of Brain Heart Infusion broth
1 milliliter of stationary phase Enterococcus faecalis in Brain Heart Infusion broth
In a 2 mL tube, combine the BHI broth and the bacteria culture.
Shake the tube on a vortexer
Bake for 2 hours at 37°C
Do the dishes. There's always dishes to be done
Do your homework. There's always homework to be done.
:lulz: I particularly enjoy your not-so-subtle reference to Shake-&-Bake.
Quote from: Mesozoic Mister Nigel on February 07, 2017, 06:59:13 PM
:lulz: I particularly enjoy your not-so-subtle reference to Shake-&-Bake.
:thanks:
Day 1 Part 2:
Water Sample Incubation
Ingredients:
5 mL of septic water
5 mL of BHI Broth
1 mL of Log Phase Enterococcus from Part 1
Gently agitate tube of septic water by hand. Vortexing too vigorously might knock the little feet off phage present. In 15 mL tube, combine BHI broth, septic water, and log phase bacteria. Note that chunks of poop will block up the pipette. Slowly pipette back into sample to remove blockage. Don't jam down on it unless you want to risk getting what is now 2 month old poop water all over the prep area. When all ingredients have been combined bake for 8 hours at 37°C.*
*I ain't staying here til 10:45 pm, so when I leave around 8 pm, I'm going to remove the tubes from the incubator and leave them in my drawer overnight.
Finally a recipe that looks edible.
Quote from: Xaz on February 07, 2017, 09:34:38 PM
Finally a recipe that looks edible.
Just you wait until Friday when I'm finally done