Cooking With Twid: How to Make Bacteriophage Lysate (Hopefully)

[Nephew Twiddleton]

Day 1 Part 1:
Prepare Log Phase Bacteria

Ingredients:
20 microliters of Brain Heart Infusion broth
1 milliliter of stationary phase Enterococcus faecalis in Brain Heart Infusion broth

In a 2 mL tube, combine the BHI broth and the bacteria culture.
Shake the tube on a vortexer
Bake for 2 hours at 37°C

Do the dishes. There's always dishes to be done
Do your homework. There's always homework to be done.

[Mesozoic Mister Nigel]

  I particularly enjoy your not-so-subtle reference to Shake-&-Bake.

[Nephew Twiddleton]

I particularly enjoy your not-so-subtle reference to Shake-&-Bake.

 

[Nephew Twiddleton]

Day 1 Part 2:
Water Sample Incubation

Ingredients:
5 mL of septic water
5 mL of BHI Broth
1 mL of Log Phase Enterococcus from Part 1

Gently agitate tube of septic water by hand. Vortexing too vigorously might knock the little feet off phage present. In 15 mL tube, combine BHI broth, septic water, and log phase bacteria. Note that chunks of poop will block up the pipette. Slowly pipette back into sample to remove blockage. Don't jam down on it unless you want to risk getting what is now 2 month old poop water all over the prep area. When all ingredients have been combined bake for 8 hours at 37°C.*

*I ain't staying here til 10:45 pm, so when I leave around 8 pm, I'm going to remove the tubes from the incubator and leave them in my drawer overnight.

[Vanadium Gryllz]

Finally a recipe that looks edible.

[Nephew Twiddleton]

Finally a recipe that looks edible.

Just you wait until Friday when I'm finally done