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« on: June 21, 2017, 12:59:32 am »
So I kinda feel like my advisor is off the deep end.
She wants preliminary results for a project she's been sitting on for three years but we just started this Fall. She wants these results in a month. She wants me to figure out from scratch with no guidance how to use imaging software no one else in the lab knows how to use, that was designed for immunofluorescence, to quantify IHC-DAB. She gave me about four days to figure this out, and to perform a validation experiment using tissue from a previous grad student who is gone. Going through that student's data, I already can see and know beyond a shadow of a doubt that the previous data, which has been accepted for publication, is bad. At least the cell counts for the supraoptic nucleus are bad. I don't have time to deal with this and it's also not my problem, but I have a feeling it is about to become my problem on top of having three weeks to process and assay 124 brains.
The reason she has a problem with bad data is because her approach to training is to throw a project at a student and tell them to figure it out, just as she is doing with me right now. If she had taken 20 minutes to train the last student, they would have known that they were counting cells in a region caudal to the supraoptic nucleus, and this data would not be bad. I can correctly quantify staining in these tissues, but I can't use these tissues to validate my method because the existing data is bad... this is a waste of my time. And everyone else's time.