Principia Discordia

Principia Discordia => Discordian Recipes => Topic started by: Nephew Twiddleton on February 07, 2017, 06:29:02 pm

Title: Cooking With Twid: How to Make Bacteriophage Lysate (Hopefully)
Post by: Nephew Twiddleton on February 07, 2017, 06:29:02 pm
Day 1 Part 1:
Prepare Log Phase Bacteria

Ingredients:
20 microliters of Brain Heart Infusion broth
1 milliliter of stationary phase Enterococcus faecalis in Brain Heart Infusion broth

In a 2 mL tube, combine the BHI broth and the bacteria culture.
Shake the tube on a vortexer
Bake for 2 hours at 37C

Do the dishes. There's always dishes to be done
Do your homework. There's always homework to be done.
Title: Re: Cooking With Twid: How to Make Bacteriophage Lysate (Hopefully)
Post by: Mesozoic Mister Nigel on February 07, 2017, 06:59:13 pm
 :lulz: I particularly enjoy your not-so-subtle reference to Shake-&-Bake.

Title: Re: Cooking With Twid: How to Make Bacteriophage Lysate (Hopefully)
Post by: Nephew Twiddleton on February 07, 2017, 07:18:44 pm
:lulz: I particularly enjoy your not-so-subtle reference to Shake-&-Bake.

  :thanks:
Title: Re: Cooking With Twid: How to Make Bacteriophage Lysate (Hopefully)
Post by: Nephew Twiddleton on February 07, 2017, 08:19:26 pm
Day 1 Part 2:
Water Sample Incubation

Ingredients:
5 mL of septic water
5 mL of BHI Broth
1 mL of Log Phase Enterococcus from Part 1

Gently agitate tube of septic water by hand. Vortexing too vigorously might knock the little feet off phage present. In 15 mL tube, combine BHI broth, septic water, and log phase bacteria. Note that chunks of poop will block up the pipette. Slowly pipette back into sample to remove blockage. Don't jam down on it unless you want to risk getting what is now 2 month old poop water all over the prep area. When all ingredients have been combined bake for 8 hours at 37C.*

*I ain't staying here til 10:45 pm, so when I leave around 8 pm, I'm going to remove the tubes from the incubator and leave them in my drawer overnight.
Title: Re: Cooking With Twid: How to Make Bacteriophage Lysate (Hopefully)
Post by: Vanadium Gryllz on February 07, 2017, 09:34:38 pm
Finally a recipe that looks edible.
Title: Re: Cooking With Twid: How to Make Bacteriophage Lysate (Hopefully)
Post by: Nephew Twiddleton on February 07, 2017, 10:35:06 pm
Finally a recipe that looks edible.

Just you wait until Friday when I'm finally done