At least, not in the way the question is most often posed. In many psychology books, operating on a philosophy that is straight outta 1896, you will see again and again statements like "This makes humans unique among the animals of the world". This statement is almost always unequivocally false.
There is no one thing, no great difference, that makes humans different from other animals. Nothing that is biologically derived, anyway; you could argue that no other animal wears pants, and you would probably be correct, but given Nature's history of proving us wrong, eventually we'd probably discover some small Amazonian beetle that weaves pants for its young out of caterpillar silk. Other animals have culture, other animals have language, other animals use tools, other animals have enormous frontal lobes. There is simply no one thing that is so special about humans that we can hold it up like a trophy, some sort of divine symbol that we stand apart from all the other species. In all ways, our differences are emergent and in measures of degree, using different versions of the same structures present in other animals in ways that make us unique-- just like all the other animals.
I would like to see the "What makes humans unique and different from all other animals?" question put to bed forever. It is an irrelevant question, it asks nothing useful and there is no useful or enlightening answer. Seeking one fundamental difference, something which we share with no other creature, is a philosophical and scientific dead-end; and at this point, philosophy has nowhere to go if it fails to embrace science. "What makes us different from all the other animals?" is a question as deep and as elucidating as "What makes a horse different from a badger?"
If we can't be satisfied with that, we probably aren't ready to move forward in asking the more significant question, not of what sets us apart, but of how we fit in.