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Cooking With Twid: How to Make Bacteriophage Lysate (Hopefully)

Started by Nephew Twiddleton, February 07, 2017, 06:29:02 PM

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Nephew Twiddleton

Day 1 Part 1:
Prepare Log Phase Bacteria

Ingredients:
20 microliters of Brain Heart Infusion broth
1 milliliter of stationary phase Enterococcus faecalis in Brain Heart Infusion broth

In a 2 mL tube, combine the BHI broth and the bacteria culture.
Shake the tube on a vortexer
Bake for 2 hours at 37°C

Do the dishes. There's always dishes to be done
Do your homework. There's always homework to be done.
Strange and Terrible Organ Laminator of Yesterday's Heavy Scene
Sentence or sentence fragment pending

Soy El Vaquero Peludo de Oro

TIM AM I, PRIMARY OF THE EXTRA-ATMOSPHERIC SIMIANS

Mesozoic Mister Nigel

 :lulz: I particularly enjoy your not-so-subtle reference to Shake-&-Bake.

"I'm guessing it was January 2007, a meeting in Bethesda, we got a bag of bees and just started smashing them on the desk," Charles Wick said. "It was very complicated."


Nephew Twiddleton

Strange and Terrible Organ Laminator of Yesterday's Heavy Scene
Sentence or sentence fragment pending

Soy El Vaquero Peludo de Oro

TIM AM I, PRIMARY OF THE EXTRA-ATMOSPHERIC SIMIANS

Nephew Twiddleton

Day 1 Part 2:
Water Sample Incubation

Ingredients:
5 mL of septic water
5 mL of BHI Broth
1 mL of Log Phase Enterococcus from Part 1

Gently agitate tube of septic water by hand. Vortexing too vigorously might knock the little feet off phage present. In 15 mL tube, combine BHI broth, septic water, and log phase bacteria. Note that chunks of poop will block up the pipette. Slowly pipette back into sample to remove blockage. Don't jam down on it unless you want to risk getting what is now 2 month old poop water all over the prep area. When all ingredients have been combined bake for 8 hours at 37°C.*

*I ain't staying here til 10:45 pm, so when I leave around 8 pm, I'm going to remove the tubes from the incubator and leave them in my drawer overnight.
Strange and Terrible Organ Laminator of Yesterday's Heavy Scene
Sentence or sentence fragment pending

Soy El Vaquero Peludo de Oro

TIM AM I, PRIMARY OF THE EXTRA-ATMOSPHERIC SIMIANS

Vanadium Gryllz

"I was fine until my skin came off.  I'm never going to South Attelboro again."

Nephew Twiddleton

Strange and Terrible Organ Laminator of Yesterday's Heavy Scene
Sentence or sentence fragment pending

Soy El Vaquero Peludo de Oro

TIM AM I, PRIMARY OF THE EXTRA-ATMOSPHERIC SIMIANS